Journal: Cell Proliferation
Article Title: Effects of neurotrophin receptor‐mediated MAGE homology on proliferation and odontoblastic differentiation of mouse dental pulp cells
doi: 10.1111/cpr.12171
Figure Lengend Snippet: Expression of NRAGE during odontoblastic differentiation of mDPCs. mRNA expression of mineralization‐related markers and NRAGE on days 0, 7 and 14 after induction: (a) DMP1, (b) DSPP, (c) ALP, (d) ALP activity and (e) NRAGE. Glyceraldehyde‐3‐phosphate (GAPDH) was used as normalization control. (f) DSP, DMP1 and NRAGE protein levels on days 0, 7 and 14 after induction. *Significant difference (P < 0.05) versus day 0, **Significant difference (P < 0.01) versus day 0.
Article Snippet: After blocking, primary antibodies to mouse anti‐mouse NRAGE (Santa Cruz Biotechnology Inc., Dallas, TX, USA), rabbit anti‐mouse DMP1 (Bio Vision Research Products, Mountain View, CA, USA), rabbit anti‐mouse DSP (Santa Cruz Biotechnology Inc), rabbit anti‐mouse nuclear factor‐κB (NF‐κB) (Epitomics, Inc., Burlingame, CA, USA) and rabbit anti‐mouse β‐actin (Santa Cruz Biotechnology, Inc) were used.
Techniques: Expressing, Activity Assay